The 3D Facility Cellomics microscope is a high capacity, high throughput & fully automated single cell epifluorescent microscopy platform located in a full Biosafety level 2 room. This powerful instrument can image up to 6 channels (including bright-field) allowing complex multiplexing and acquisition of an array of biological parameters at once.
The facility manager provide full assistance to develop any high throughput assays that the facility user would require.
Instrument details:
• State of the art Zeiss Oberver Z1 epifluorescent microscope
• 4 Powerful color LED light engine source for live multicolor fluorescent experiments (Excitation: 386nm, 485nm, 549nm, 650nm)
• Versatile fluorescence imaging with the emission filters ranging from 500 – 750nm
• Transmitted light pictures with the removable brightfield module
• Temperature & CO2 control environment in the Live cell chamber allows study of parameters over time
• Automatic loading of plate stacks with the CRS CataLyst Express robotic arm
• Automatic focusing with proprietary focusing system
• Advanced data visualization & analysis with the Cellomics Scan and View software
Applications:
• Analysis of cell structure and organelles
• Cell viability (1)
• Proliferation (1)
• Apoptosis (1, 2)
• Autophagy (3, 4)
• Mitochondrial health (5)
• Mitotic index (6)
• Oxidative stress (7)
• DNA damage (8)
• Phospholipidosis and steatosis (9)
• Endocytosis (10)
And many more…
(1) White JP, Billin AN, Campbell ME, Russell AJ, Huffman KM, Kraus WE. Stem Cell Reports. 2018 Aug 14;11(2):425-439.
(2) Prentice KJ, Luu L, Allister EM, Liu Y, Jun LS, Sloop KW, Hardy AB, Wei L, Jia W, Fantus IG, Sweet DH, Sweeney G, Retnakaran R, Dai FF, Wheeler MB. Cell Metab. 2014 Apr 1;19(4):653-66.
(3) Jiang P, Mizushima N (2014) Cell Res 24:69–79.
(4) Joachim J, Jiang M, McKnight NC et al. (2015) Methods 75:96–104.
(5) Abraham VC, Towne DL, Waring JF, Warrior U, Burns DJ. J Biomol Screen. 2008 Jul;13(6):527-37.
(6) Gasparri F, Mariani M, Sola F, Galvani A. J Biomol Screen. 2004 Apr;9(3):232-43.
(7) Cano M, Wang L, Wan J, Barnett BP, Ebrahimi K, Qian J, Handa JT. Free Radic Biol Med. 2014 Apr;69:1-14.
(8) Garcia-Canton C, Anadon A, Meredith C. Mutat Res. 2013 Oct 9;757(2):158-66.
(9) Morelli JK, Buehrle M, Pognan F, Barone LR, Fieles W, Ciaccio PJ. Cell Biol Toxicol. 2006 Jan;22(1):15-27.
(10) Ghosh RN, DeBiasio R, Hudson CC, Ramer ER, Cowan CL, Oakley RH. J Biomol Screen. 2005 Aug;10(5):476-84.