Nikon Swept Field (SFC) and TIRF confocal microscope

Left: SFC/TIRF microscope; bottom right: bovine pulmonary artery cell stained for nucleus (bleu), mitochondria (red) and actin (green); top right: laser launch. Source-3D Facility

The 3D Facility SFC Microscope enables dynamic real time live cell imaging with a high-sensitivity & high-speed confocal fluorescence microscope in a full Biosafety level 2 room. The slit scanning mode of the SFC microscope produces faster acquisition and lower laser driven toxicity. The TIRF module allows imaging of the surface of the cells with a very low signal-to-noise compared to epifluorescence of confocal microscopes.

Instrument details:  
• Powerful laser lines: 405nm, 488nm, 561nm, 640nm
• Motorized components for automated acquisition
• Live cell chamber: temperature & CO2 control environment
• Easy to use: z-stack, multipoint, large image XY stitching applications for ultra-high definition, time-lapse imaging automated cell counting, fluorescence intensity analysis, morphometric measurements
• Real time confocal imaging at speed up to 30 fps with the SFC module
• Nikon perfect focus to minimize focus drift during time lapse acquisition
• Full integration with NIS Elements software makes the SFC and TIRF modules very easy to learn and use

3D real time imaging
• volume visualization & analysis studies
• Live cell imaging
• Well plate scanning
• Cell counting
• Cell viability, proliferation, autophagy, cytotoxicity, metabolism
• Cell structure
• Calcium transient studies
• Fast neural events
• Colocalization studies
• Cell surface event tracking, localization & colocalization

And many more….

3D rendering of intestinal organoid cells stained for nucleus (green) and cell proliferation (yellow, Edu). Source – Dr. Patricia Brubaker, University of Toronto.